Shaping the Future of Sustainable Hydrogen Production
The PhotoSynH2 consortium is on a mission to transform the landscape of renewable energy through a series of ambitious, yet meticulously planned goals. Our journey is guided by the pursuit of innovation, with the aim to refine and enhance the process of hydrogen production using the power of nature itself. Here’s how we plan to make a difference:

Engineering Precision
Our first step involves harnessing the potential of computational design to tailor CRISPRi regulation, proteins, and riboregulators with specific functions. This precise genetic engineering aims to create a foundation for targeted enhancements in our cyanobacteria, setting the stage for groundbreaking advancements in hydrogen production.

Evolutionary Optimisation
We’re not just stopping at genetic engineering. Our consortium is dedicated to continuously refining our engineered systems through evolutionary optimisation. This includes perfecting gene regulators and hydrogenases, as well as devices that consume oxygen to maximise hydrogen output. Such optimisation ensures that our systems are not only efficient but also resilient and adaptable to various conditions.

Adapting to Scale
A pivotal goal is to adapt the genetically engineered Synechocystis strain to thrive within the conditions of a large-scale bioreactor. This step is crucial for transitioning from laboratory-scale experiments to real-world application, enabling us to produce hydrogen on a scale that can make a tangible impact.

Enhancing Photosynthesis
By optimising photosynthesis and directing electron flow more effectively, we aim to significantly boost hydrogen production in cyanobacteria. Our efforts include modifying the photosynthetic antenna to increase light tolerance and broaden the spectrum of usable light, thereby enhancing the efficiency and resilience of our cyanobacterial system.

Harnessing Novel Enzymes
Identifying and optimising novel [FeFe]-hydrogenase enzymes with an improved capacity for hydrogen production is another cornerstone of our project. Through both discovery and rational design, we aim to unlock new potentials for cyanobacterial hydrogen production, pushing the boundaries of what’s currently achievable.

Environmental Adaptation
Acknowledging the importance of sustainability, we are working to improve Synechocystis growth and salt tolerance. This forward-thinking approach allows for the possibility of using seawater in cultivation processes, reducing freshwater dependency and making our technology more accessible and environmentally friendly.

Oxygen Management
To further refine our system, we’re developing strategies to reduce intracellular oxygen levels. This involves implementing hydrogen production and oxygen consumption devices into our engineered chassis, creating a more efficient and effective environment for hydrogen generation.

Photobioreactor Innovation
Finally, we are committed to improving the design, construction, and performance of a custom multiplate photobioreactor (MPBR). This reactor, designed to harness solar light for photobiological hydrogen production, represents the culmination of our efforts to bring sustainable hydrogen production to a practical, impactful scale.

The Real Impact
Through these goals, the PhotoSynH2 consortium aims to not only advance the science behind hydrogen production but also to provide a tangible, scalable solution for clean energy. Our work promises to contribute significantly to reducing carbon footprints, enhancing energy security, and paving the way for a sustainable future. Join us as we embark on this exciting journey, where innovation meets impact, and science ushers in a new era of renewable energy.